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pk 7100 rrid ab 2336827  (Vector Laboratories)


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    Vector Laboratories pk 7100 rrid ab 2336827
    Pk 7100 Rrid Ab 2336827, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pk+7100/VECTASTAIN+Elite+ABC+HRP+Reagent%2C+R%2ET%2EU%2E+(Peroxidase%2C+Ready-to-Use)/pm41957073-94-23-25
    Average 96 stars, based on 2275 article reviews
    pk 7100 rrid ab 2336827 - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Cytomegalovirus infection is common in prostate cancer and antiviral therapies inhibit progression in disease models.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Reagent, R.T.U. (PK-7100, Vector laboratories) 30 min, after which slides were washed in PBS and incubated with ImmPACT DAB (Vector Laboratories) or TSA using the Alexa Fluor 488 Tyramide SuperBoost kit (B40932, Thermo Fisher) according to the manufacturer’s instructions. ..

    Article Title: Cytomegalovirus infection is common in prostate cancer and antiviral therapies inhibit progression in disease models
    Article Snippet: After washing in PBS, slides were incubated with a secondary antibody conjugated with biotin (donkey anti‐mouse biotin or donkey anti‐rabbit biotin, 1:500, Jackson laboratories, Bar Harbor, Maine, USA) or a secondary antibody conjugated with a fluorophore (donkey anti‐mouse 488/cy3/cy5, donkey anti‐rabbit 488/cy3/cy5, Jackson laboratories). .. If incubated with a secondary antibody conjugated with biotin, slides were then incubated with VECTASTAIN Elite ABC HRP Reagent, R.T.U. (PK‐7100, Vector laboratories) 30 min, after which slides were washed in PBS and incubated with ImmPACT DAB (Vector Laboratories) or TSA using the Alexa Fluor 488 Tyramide SuperBoost kit ( B40932 , Thermo Fisher) according to the manufacturer's instructions. ..

    Article Title: Generation and characterization of cerebellar granule neurons specific knockout mice of Golli-MBP
    Article Snippet: .. The sections were incubated with VECTASTAIN Elite ABC Reagent, Peroxidase, R.T.U. (#PK-7100, Vector Laboratories) for 30 min at room temperature, following which DAB solution was applied. ..

    Article Title: Increasing dietary nutrient levels modulates colon immune adaptation and alleviates inflammation in the epithelial heterogeneous nuclear ribonucleoprotein I (Hnrnp I) knockout mice.
    Article Snippet: Image analysis was performed using Image J software (Rasband, W.S., ImageJ, U. S. National Institutes of Health, http://imagej.nih.gov/ij/ , 1997–2017). .. Sections used for IHC staining were deparaffined and hydrated, following the same procedures as in “histological analysis.” Then, sections were blocked with blocking buffers (1X PBS/1% bovine serum albumin/5% normal goat serum/0.05% Triton X-100) and incubated with antibodies following the respective procedures: primary anti-CD4, Clone 4SM95 (1:100, Invitrogen by ThermoFisher Scientific, Cat#14-9766-82) overnight at 4 °C; after the primary incubation, sections were washed with 1X PBS 3 times (5 minutes each time), then incubated with anti-rat IgG secondary antibodies (1:20 0, BA-940 0, Vector Laboratories, Inc., Burlingame, CA) at room temperature for 30 minutes; after primary and secondary incubation, all slides were rewashed in 1X PBS and incubated with Vectastain Elite ABC HRP reagents, R.T.U (PK-7100, Vector Laboratories, Inc., Burlingame, CA) for 30 minutes; finally, sections were stained with DAB peroxidase (HRP) substrate Kit (SK-4100, Vector Laboratories) in the dark at room temperature for 1–2 minutes depending on color development of negative control samples. .. Next, slides were washed with 1X PBS, counter-stained with hematoxylin, mounted with Permount (SP15-500, ThermoFisher Scientific), and dried overnight.

    Article Title: Sodium Accumulation and Blood Capillary Rarefaction in the Skin Predispose Spontaneously Hypertensive Rats to Salt Sensitive Hypertension.
    Article Snippet: .. The CD31 antibody was detected by biotinylated goat anti-rabbit IgG (H + L) (BA-1000-1.5, Vector Laboratories, Burlingame, CA, USA, dilution 1:200), after which the sections were incubated with VECTASTAIN® Elite ABC-HRP Reagent, Peroxidase, R.T.U. (PK-7100, Vector Laboratories) for 30 min. .. The Simple Stain MAX PO (MULTI) Universal Immuno-peroxidase Polymer anti-mouse, anti-rabbit Histofine (414152F, Nichirei Biosciences, Tokyo, Japan) was used to detect the primary anti-podoplanin antibody.

    Article Title: Generation and characterization of cerebellar granule neurons specific knockout mice of Golli-MBP.
    Article Snippet: .. The sections were incubated with VECTASTAIN Elite ABC Reagent, Peroxidase, R.T.U. (#PK-7100, Vector Laboratories) for 30 min at room temperature, following which DAB solution was applied. ..

    Immunohistochemistry:

    Article Title: Increasing dietary nutrient levels modulates colon immune adaptation and alleviates inflammation in the epithelial heterogeneous nuclear ribonucleoprotein I (Hnrnp I) knockout mice.
    Article Snippet: Image analysis was performed using Image J software (Rasband, W.S., ImageJ, U. S. National Institutes of Health, http://imagej.nih.gov/ij/ , 1997–2017). .. Sections used for IHC staining were deparaffined and hydrated, following the same procedures as in “histological analysis.” Then, sections were blocked with blocking buffers (1X PBS/1% bovine serum albumin/5% normal goat serum/0.05% Triton X-100) and incubated with antibodies following the respective procedures: primary anti-CD4, Clone 4SM95 (1:100, Invitrogen by ThermoFisher Scientific, Cat#14-9766-82) overnight at 4 °C; after the primary incubation, sections were washed with 1X PBS 3 times (5 minutes each time), then incubated with anti-rat IgG secondary antibodies (1:20 0, BA-940 0, Vector Laboratories, Inc., Burlingame, CA) at room temperature for 30 minutes; after primary and secondary incubation, all slides were rewashed in 1X PBS and incubated with Vectastain Elite ABC HRP reagents, R.T.U (PK-7100, Vector Laboratories, Inc., Burlingame, CA) for 30 minutes; finally, sections were stained with DAB peroxidase (HRP) substrate Kit (SK-4100, Vector Laboratories) in the dark at room temperature for 1–2 minutes depending on color development of negative control samples. .. Next, slides were washed with 1X PBS, counter-stained with hematoxylin, mounted with Permount (SP15-500, ThermoFisher Scientific), and dried overnight.

    Staining:

    Article Title: Increasing dietary nutrient levels modulates colon immune adaptation and alleviates inflammation in the epithelial heterogeneous nuclear ribonucleoprotein I (Hnrnp I) knockout mice.
    Article Snippet: Image analysis was performed using Image J software (Rasband, W.S., ImageJ, U. S. National Institutes of Health, http://imagej.nih.gov/ij/ , 1997–2017). .. Sections used for IHC staining were deparaffined and hydrated, following the same procedures as in “histological analysis.” Then, sections were blocked with blocking buffers (1X PBS/1% bovine serum albumin/5% normal goat serum/0.05% Triton X-100) and incubated with antibodies following the respective procedures: primary anti-CD4, Clone 4SM95 (1:100, Invitrogen by ThermoFisher Scientific, Cat#14-9766-82) overnight at 4 °C; after the primary incubation, sections were washed with 1X PBS 3 times (5 minutes each time), then incubated with anti-rat IgG secondary antibodies (1:20 0, BA-940 0, Vector Laboratories, Inc., Burlingame, CA) at room temperature for 30 minutes; after primary and secondary incubation, all slides were rewashed in 1X PBS and incubated with Vectastain Elite ABC HRP reagents, R.T.U (PK-7100, Vector Laboratories, Inc., Burlingame, CA) for 30 minutes; finally, sections were stained with DAB peroxidase (HRP) substrate Kit (SK-4100, Vector Laboratories) in the dark at room temperature for 1–2 minutes depending on color development of negative control samples. .. Next, slides were washed with 1X PBS, counter-stained with hematoxylin, mounted with Permount (SP15-500, ThermoFisher Scientific), and dried overnight.

    Blocking Assay:

    Article Title: Increasing dietary nutrient levels modulates colon immune adaptation and alleviates inflammation in the epithelial heterogeneous nuclear ribonucleoprotein I (Hnrnp I) knockout mice.
    Article Snippet: Image analysis was performed using Image J software (Rasband, W.S., ImageJ, U. S. National Institutes of Health, http://imagej.nih.gov/ij/ , 1997–2017). .. Sections used for IHC staining were deparaffined and hydrated, following the same procedures as in “histological analysis.” Then, sections were blocked with blocking buffers (1X PBS/1% bovine serum albumin/5% normal goat serum/0.05% Triton X-100) and incubated with antibodies following the respective procedures: primary anti-CD4, Clone 4SM95 (1:100, Invitrogen by ThermoFisher Scientific, Cat#14-9766-82) overnight at 4 °C; after the primary incubation, sections were washed with 1X PBS 3 times (5 minutes each time), then incubated with anti-rat IgG secondary antibodies (1:20 0, BA-940 0, Vector Laboratories, Inc., Burlingame, CA) at room temperature for 30 minutes; after primary and secondary incubation, all slides were rewashed in 1X PBS and incubated with Vectastain Elite ABC HRP reagents, R.T.U (PK-7100, Vector Laboratories, Inc., Burlingame, CA) for 30 minutes; finally, sections were stained with DAB peroxidase (HRP) substrate Kit (SK-4100, Vector Laboratories) in the dark at room temperature for 1–2 minutes depending on color development of negative control samples. .. Next, slides were washed with 1X PBS, counter-stained with hematoxylin, mounted with Permount (SP15-500, ThermoFisher Scientific), and dried overnight.

    Negative Control:

    Article Title: Increasing dietary nutrient levels modulates colon immune adaptation and alleviates inflammation in the epithelial heterogeneous nuclear ribonucleoprotein I (Hnrnp I) knockout mice.
    Article Snippet: Image analysis was performed using Image J software (Rasband, W.S., ImageJ, U. S. National Institutes of Health, http://imagej.nih.gov/ij/ , 1997–2017). .. Sections used for IHC staining were deparaffined and hydrated, following the same procedures as in “histological analysis.” Then, sections were blocked with blocking buffers (1X PBS/1% bovine serum albumin/5% normal goat serum/0.05% Triton X-100) and incubated with antibodies following the respective procedures: primary anti-CD4, Clone 4SM95 (1:100, Invitrogen by ThermoFisher Scientific, Cat#14-9766-82) overnight at 4 °C; after the primary incubation, sections were washed with 1X PBS 3 times (5 minutes each time), then incubated with anti-rat IgG secondary antibodies (1:20 0, BA-940 0, Vector Laboratories, Inc., Burlingame, CA) at room temperature for 30 minutes; after primary and secondary incubation, all slides were rewashed in 1X PBS and incubated with Vectastain Elite ABC HRP reagents, R.T.U (PK-7100, Vector Laboratories, Inc., Burlingame, CA) for 30 minutes; finally, sections were stained with DAB peroxidase (HRP) substrate Kit (SK-4100, Vector Laboratories) in the dark at room temperature for 1–2 minutes depending on color development of negative control samples. .. Next, slides were washed with 1X PBS, counter-stained with hematoxylin, mounted with Permount (SP15-500, ThermoFisher Scientific), and dried overnight.



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